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Image Search Results
Journal: The Journal of Biological Chemistry
Article Title: Temporal Interleukin-1β Secretion from Primary Human Peripheral Blood Monocytes by P2X7-independent and P2X7-dependent Mechanisms
doi: 10.1074/jbc.M109.072793
Figure Lengend Snippet: Stimulation of monocytes for 24 h with specific TLR agonists results in P2X7 receptor-independent IL-1β release. Peripheral blood monocytes treated for 24 h with 1 ng/ml LPS ( A ), 1 μg/ml CL075 ( B ), 10 μg/ml gardiquimod ( C ), or 1 μg/ml CL097 ( D ) released IL-1β as measured in supernatants by ELISA, whereas 0.1 μg/ml CL097 did not cause detectable IL-1β secretion ( E ). IL-1β release was not significantly inhibited by the presence of 15d (A438079) P2X7 receptor antagonist, KN62, or DMSO vehicle control. The data are the means ± S.E. for n = 3–5 separate donors.
Article Snippet:
Techniques: Enzyme-linked Immunosorbent Assay